Multinuclear forms in a dyskinetoplastic strain of Trypanosoma evansi in mice.

Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections

BACKGROUND: Based on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region lacking homology to other known VSG genes. A total of 39 different trypanosome stocks were tested using the RoTat 1.2 based Polymerase Chain Reaction (PCR). RESULTS: This PCR yielded a 205 bp product in ...

Evidence for the Retention of Kinetoplast Dna in an Acriflavine-induced Dyskinetoplastic Strain of Trypanosoma Brucei Which Replicates the Altered Central Element of the Kinetoplast

A pleomorphic dyskinetoplastic strain of Trypanosoma brucei was produced by repeated acriflavine treatment. No kinetoplastic cells reappeared after 2 yr of maintenance in the absence of acriflavine. These dyskinetoplastic cells retained and therefore replicated the central element of the kinetoplast. This element was present in the "condensed" state typical of acriflavine-treated cells rather t...

Evaluation of Humoral Immune Response to Foot-and-Mouth Disease Vaccination in Experimentally Infected Guinea Pigs with Trypanosoma evansi

Evolution of dyskinetoplastic trypanosomes: how, and how often?

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New Trypanosoma evansi Type B Isolates from Ethiopian Dromedary Camels.

BACKGROUND Trypanosoma (T.) evansi is a dyskinetoplastic variant of T. brucei that has gained the ability to be transmitted by all sorts of biting flies. T. evansi can be divided into type A, which is the most abundant and found in Africa, Asia and Latin America and type B, which has so far been isolated only from Kenyan dromedary camels. This study aimed at the isolation and the genetic and ph...